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Polymers 2018, 10(7), 768; https://doi.org/10.3390/polym10070768

秒速赛车走势图网址:Bioactive and Bioadhesive Catechol Conjugated Polymers for Tissue Regeneration

1
Institute of Polymer Science and Technology, ICTP-CSIC, Juan de la Cierva 3, 28006 Madrid, Spain
2
CIBER’s Bioengineering, Biomaterials and Nanomedicine, CIBER-BBN, Health Institute Carlos III, C/Monforte de Lemos 3-5, Pabellón 11, 28029 Madrid, Spain
*
Author to whom correspondence should be addressed.
Received: 7 June 2018 / Revised: 3 July 2018 / Accepted: 11 July 2018 / Published: 13 July 2018
(This article belongs to the Special Issue Polymers for Therapy and Diagnostics)
Full-Text   |   PDF [2646 KB, uploaded 13 July 2018]   |  
Graphical abstract
">
Figure 1
<p>Tridimensional diagram showing the variation of instantaneous H copolymer molar fraction as a function of conversion and H feed molar fraction. Red lines represent reaction course for H feed compositions used in this work (0.2 and 0.4 mol %).</p> ">
Figure 2
<p>Scheme of the synthesis of the acid chloride derivative of hydrocaffeic acid (HCA), VH copolymers and the catechol conjugated polymers VHC.</p> ">
Figure 3
<p>Atomic force microscopy (AFM) (<bold>left</bold>) and scanning electron microscopy (SEM) (<bold>right</bold>) images of (<bold>a</bold>) VHC2 terpolymer and (<bold>b</bold>) VHC22 terpolymer.</p> ">
Figure 4
<p>In vitro degradation kinetics of VHC films in Dulbecco’s modified Eagle’s medium (DMEM) (pH = 7.4) at 37 °C. Data are presented as mean ± standard deviation (<italic>n</italic> = 3).</p> ">
Figure 5
<p>(<bold>a</bold>) Application of the polymer solution on the porcine tissue and skin samples attached each other. (<bold>b</bold>) Comparative studies in adhesion forces between the catechol conjugated polymers VHC2 and VHC22. Each line represents the stress-displacement representative curve of the two compositions after four replicates. (<bold>c</bold>) Detachment stress of the catechol containing polymers VHC2 and VHC22. Significant differences are denoted in the graph comparing the two groups at the significance level of *** <italic>p</italic> &lt; 0.001.</p> ">
Figure 6
<p>(<bold>a</bold>) Porcine skin samples irradiated with the terpolymer film (left) and after removing the terpolymer film (right). (<bold>b</bold>) Water contact angle images of the irradiated skin under de terpolymer film (left) and of the nude irradiated skin (right). (<bold>c</bold>) Water contact angle results of the skin control (non-irradiated and irradiated) and the skin under the VHC films. Significant differences are denoted in the graph comparing the values of the irradiated samples under the VHC films and the irradiated control skin (*** <italic>p</italic> &lt; 0.001).</p> ">
Figure 7
<p>Cell viability of human bone marrow mesenchymal stem cells (hBMSCs) treated with medium extracts of VHC films taken at different times. The diagrams include the mean and the standard deviation (<italic>n</italic> = 8).</p> ">
Figure 8
<p>Intracellular reactive oxygen species (ROS) activity in hBMSCs measured from fluorescence emission at different times after treatment with VHC films extracts collected at 24 h. The diagrams include the mean, the standard deviation (<italic>n</italic> = 4) and the analysis of variance (ANOVA) between the different groups and the positive control at each time (* <italic>p</italic> &lt; 0.05, ** <italic>p</italic> &lt; 0.01, *** <italic>p</italic> &lt; 0.001).</p> ">
Figure 9
<p>Inhibitory effects of VHC terpolymers on nitric oxide production in lipopolysaccharide (LPS) stimulated RAW 264.7 cells (bars) and cellular viability (lines and symbols).</p> ">

Abstract

The effective treatment of chronic wounds constitutes one of the most common worldwide healthcare problem due to the presence of high levels of proteases, free radicals and exudates in the wound, which constantly activate the inflammatory system, avoiding tissue regeneration. In this study, we describe a multifunctional bioactive and resorbable membrane with in-built antioxidant agent catechol for the continuous quenching of free radicals as well as to control inflammatory response, helping to promote the wound-healing process. This natural polyphenol (catechol) is the key molecule responsible for the mechanism of adhesion of mussels providing also the functionalized polymer with bioadhesion in the moist environment of the human body. To reach that goal, synthesized statistical copolymers of N-vinylcaprolactam (V) and 2-hydroxyethyl methacrylate (H) have been conjugated with catechol bearing hydrocaffeic acid (HCA) molecules with high yields. The system has demonstrated good biocompatibility, a sustained antioxidant response, an anti-inflammatory effect, an ultraviolet (UV) screen, and bioadhesion to porcine skin, all of these been key features in the wound-healing process. Therefore, these novel mussel-inspired materials have an enormous potential for application and can act very positively, favoring and promoting the healing effect in chronic wounds. View Full-Text
Keywords: wound healing; catechol; conjugated; antioxidant; anti-inflammatory; bioadhesion; ultraviolet (UV) shielding wound healing; catechol; conjugated; antioxidant; anti-inflammatory; bioadhesion; ultraviolet (UV) shielding
Figures

秒速赛车是哪里的开奖 www.0dv0k.cn Graphical abstract

Graphical abstract
">
Figure 1
<p>Tridimensional diagram showing the variation of instantaneous H copolymer molar fraction as a function of conversion and H feed molar fraction. Red lines represent reaction course for H feed compositions used in this work (0.2 and 0.4 mol %).</p> ">
Figure 2
<p>Scheme of the synthesis of the acid chloride derivative of hydrocaffeic acid (HCA), VH copolymers and the catechol conjugated polymers VHC.</p> ">
Figure 3
<p>Atomic force microscopy (AFM) (<bold>left</bold>) and scanning electron microscopy (SEM) (<bold>right</bold>) images of (<bold>a</bold>) VHC2 terpolymer and (<bold>b</bold>) VHC22 terpolymer.</p> ">
Figure 4
<p>In vitro degradation kinetics of VHC films in Dulbecco’s modified Eagle’s medium (DMEM) (pH = 7.4) at 37 °C. Data are presented as mean ± standard deviation (<italic>n</italic> = 3).</p> ">
Figure 5
<p>(<bold>a</bold>) Application of the polymer solution on the porcine tissue and skin samples attached each other. (<bold>b</bold>) Comparative studies in adhesion forces between the catechol conjugated polymers VHC2 and VHC22. Each line represents the stress-displacement representative curve of the two compositions after four replicates. (<bold>c</bold>) Detachment stress of the catechol containing polymers VHC2 and VHC22. Significant differences are denoted in the graph comparing the two groups at the significance level of *** <italic>p</italic> &lt; 0.001.</p> ">
Figure 6
<p>(<bold>a</bold>) Porcine skin samples irradiated with the terpolymer film (left) and after removing the terpolymer film (right). (<bold>b</bold>) Water contact angle images of the irradiated skin under de terpolymer film (left) and of the nude irradiated skin (right). (<bold>c</bold>) Water contact angle results of the skin control (non-irradiated and irradiated) and the skin under the VHC films. Significant differences are denoted in the graph comparing the values of the irradiated samples under the VHC films and the irradiated control skin (*** <italic>p</italic> &lt; 0.001).</p> ">
Figure 7
<p>Cell viability of human bone marrow mesenchymal stem cells (hBMSCs) treated with medium extracts of VHC films taken at different times. The diagrams include the mean and the standard deviation (<italic>n</italic> = 8).</p> ">
Figure 8
<p>Intracellular reactive oxygen species (ROS) activity in hBMSCs measured from fluorescence emission at different times after treatment with VHC films extracts collected at 24 h. The diagrams include the mean, the standard deviation (<italic>n</italic> = 4) and the analysis of variance (ANOVA) between the different groups and the positive control at each time (* <italic>p</italic> &lt; 0.05, ** <italic>p</italic> &lt; 0.01, *** <italic>p</italic> &lt; 0.001).</p> ">
Figure 9
<p>Inhibitory effects of VHC terpolymers on nitric oxide production in lipopolysaccharide (LPS) stimulated RAW 264.7 cells (bars) and cellular viability (lines and symbols).</p> ">
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Puertas-Bartolomé, M.; Vázquez-Lasa, B.; San Román, J. Bioactive and Bioadhesive Catechol Conjugated Polymers for Tissue Regeneration. Polymers 2018, 10, 768.

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